In vitro multiplication of wild Manihot species with different naphthaleneacetic acid and benzylaminopurine concentrations

In vitro multiplication is an important tissue culture technique that capable of efficiently producing seedlings at any scale. It a propagation method based on the aseptic small propagules in suitable medium to enable plant regeneration. Multiplication experiments conducted set protocols adapted wild Manihot species have used modified mineral salts and MS vitamins as basic medium. Here, 25 treatments combinations regulators benzylaminopurine (BAP) naphthaleneacetic acid (NAA) 0, 0.025, 0.05, 0.075, 0.1 mg L-1 were for three genotypes (M. violaceae Pohl Müll. Arg., M. pseudoglaziovii Pax & Hoff., flabellifolia Pohl). Plant height number 1 cm minicuttings, roots, shoots, green leaves senescent recorded 120 days after explant inoculation. Pohl. Arg. presented favorable results with 0.05 0.025 NAA, respectively. Culture lacking NAA BAP favored growth Hoff.